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KMID : 0366220060410040250
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Korean Journal of Hematology
2006 Volume.41 No. 4 p.250 ~ p.258
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Survival and Graft versus Host Disease in Murine MHC Mismatched Hematopoietic Stem Cell Transplantation with Co-injection of Mesenchymal Stem Cells
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Choi Sang-Rhim
Lee Dae-Hyeoung
Jeong Chang-Mo
Hwang Hui-Sung
Chung Nack-Gyun
Cho Bin
Han Chi-Hwa
Kim Hack-Ki
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Abstract
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Background: Intravenous injection of mesenchymal and hematopoietic stem cells (MSCs, HSCs) has the disadvantages of low delivery rate to bone marrow and sequestration of cells in the lung and liver. This study was designed to determine whether there is a relationship between the administration route and dosage of stem cells and GVHD and survival.
Methods: MSCs were retrieved from five subcultured C3H/10T1/2, cell lines from C3H/He mice. HSCs were transplanted by injecting 1×107 of bone marrow mononuclear cells and 5×106 of spleen cells from six to eight week old female C3H/He mice into six week old irradiated female BALB/c mice. The groups were divided into intravenous injection (IV) and intra-marrow (IM) injection groups. IV and IM+MSC groups consisted of mice transplanted with the same bone marrow mononuclear cells and SP, IV and IM groups, with the additional co-injection of 1×106 MSCs.
Results: Evaluation of all mice, in both groups, showed no difference in GVHD and survival. However, high dose injection with 1×106 MSCs led to a decreased incidence of GVHD (P＜0.05) and improved survival (P＜0.01) in both groups.
Conclusion: The results of this study showed that the positive effects of MSC on GVHD and survival were primarily dependent on the number of injected cells.
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KEYWORD
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Graft versus host disease, Mesenchymal stem cell, Route, Dosage
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